| Factors |
Parameters |
| Forward Extraction |
|
| AOT |
20 mM |
| pH |
7.0 |
| Time |
30 min |
| Temperature |
4°C |
| Backward Extraction |
|
| Salt concentration |
Optimum |
| pH |
Optimum |
| Isobutyl alcohol |
15% v/v alcohol or 15% v/v distilled water |
| Time |
1 hr |
| Temperature |
4°C |
No. of replicates=3
Total no. of runs= 6 |