Enzyme Concentration (%) Hydrolysis Time (h) Protein Yield
Whole Fish Head Frame FTSG
(g) (%) (g) (%) (g) (%) (g) (%)
0.5 1 3.53 ± 0.15 7.06 3.28 ± 0.03 6.56 3.52 ± 0.11 7.04 2.48 ± 0.06 4.96
2 3.86 ± 0.12 7.72 3.55 ± 0.02 7.10 3.97 ± 0.01 7.94 2.75 ± 0.04 5.50
3 4.53 ± 0.11 9.06 3.75 ± 0.04 7.50 4.19 ± 0.02 8.38 2.95 ± 0.03 5.90
4 5.21 ± 0.01 10.42 3.82 ± 0.02 7.64 4.39 ± 0.03 8.78 3.28 ± 0.02 6.56
1 1 3.67 ± 0.03 7.34 3.22 ± 0.02 6.44 3.81 ± 0.04 7.62 2.96 ± 0.02 5.92
2 4.17 ± 0.10 8.34 3.53 ± 0.02 7.06 4.25 ± 0.02 8.50 3.37 ± 0.05 6.74
3 5.14 ± 0.12 10.28 3.75 ± 0.02 7.50 4.46 ± 0.02 8.92 3.58 ± 0.04 7.16
4 5.54 ± 0.01 11.08 3.92 ± 0.03 7.84 4.68 ± 0.02 9.36 3.78 ± 0.02 7.56
2 1 4.24 ± 0.11 8.48 3.39 ± 0.02 6.78 3.63 ± 0.02 7.26 3.45 ± 0.04 6.90
2 4.86 ± 0.05 9.72 4.00 ± 0.09 8.00 4.71 ± 0.03 9.42 3.95 ± 0.03 7.90
3 5.44 ± 0.02 10.88 4.22 ± 0.02 8.44 4.97 ± 0.05 9.94 4.14 ± 0.02 8.28
4 5.94 ± 0.02 11.88 4.36 ± 0.03 8.72 5.28 ± 0.02 10.56 4.26 ± 0.02 8.52
Sample size= 50 g Whole fish = 7.78% (15.57%) Head =6.15% (12.30%) Frame = 7.08% (14.16%) and Fin, tail, skin and gut (FTSG) = 6.09% (12.18%)
Table 3: Protein yield.