Figure 4: Reusability of the immobilized cells. Reaction conditions: 5 mg cells, 100 mM sodium phosphate buffer (pH 6), at 30°C. After each run, the immobilized cells were separated by filtration and the free cells were separated by centrifugation (10,000 rpm), washed with phosphate buffer (pH 6.0) for 2 times. The initial activities of the free and immobilized cells were defined as 100%.