Figure 1: Schematic illustration of colorimetric and luminescent ELISA performed. Both ELISA methods are performed on the same 96-well plate and following the same procedure of HCPs capturing by coated anti-HCP antibodies, primary detection by biotinylated anti-HCP antibodies, and secondary detection by NeutrAvidin-HRP conjugates. The signal detection is either obtained by measuring absorbance at 450 nm generated from catalyzing TMB substrate (left) or by measuring the luminescence at 425 nm generated from oxidizing of ELISA Femto substrate (right).