Detection method Log-log fit curve range (R2) LLOD (ng/ml) LLOQ (ng/ml)
Colorimetric 0.91-222.22 ng/ml (0.992) 0.819 2.483
Luminescent 0.91-222.22 ng/ml (0.998) 0.365 1.216
Luminescent 0.31-2000 ng/ml (0.991) 0.245 0.816
Table 4: The LLOD and LLOQ for luminescent and colorimetric ELISA.