Variable Levels -1 0 1 Biosurfactant (% v/v) 0.10 0.25 0.40 Enzyme pool (% v/v) 3.8 5.0 6.2 T (°C) 34 40 46 Average and standard deviations of three samples of collected effluent

Table 2: Experimental design 23 type, having as variables the temperature, LEP and biosurfactant concentrations. Conditions used in the pretreatment with enzyme pool and biosurfactant.