Condition LEP (% v/v) BS (% v/v) T (°C) Free acids (μmol/ml)*
1 3.8 0.10 34 8.73
2 6.2 0.10 34 10.07
3 3.8 0.40 34 6.81
4 6.2 0.40 34 13.97
5 3.8 0.10 46 10.94
6 6.2 0.10 46 13.29
7 3.8 0.40 46 6.00
8 6.2 0.40 46 8.41
9 5.0 0.25 40 7.2
10 5.0 0.25 40 6.97
11 5.0 0.25 40 6.92
*Difference between sample and control after 8h. Standard deviation less than 5%
Table 4: Conditions (experimental design) and results of the pretreatment with enzyme pool (LEP) and biosurfactant (BS).