Bioproduct Source System and Composition (%w/w) Kp Yield% TLL% pH Reference
Serine Protease Mango Peel PEG8000/ phosphate (-/ 4.5%) 84.2 97.3 17.2 7.5 [12]
Inverts Tomato PEG 3000/ Na2SO4 (15/12%) +5% KCl 1.1 90 - 4.5 [11]
Serine Protease Kesinai (Streblus asper) PEG6000/rich-MgSO4 (16/ 15%) - 96.7 - 7.0 [62]
Papain Papaya PEG6000/ (NH4)2SO4 (8/ 15%) - 89.9 - 5.0 [23]
Phytase Aspergillus  niger PEG6000+8000/ Citrate (10.5/ 20.5%) 0.96 96.0 - 5.6 [13]
Protease Tuna (Thunnus alalunga) PEG2000/MgSO4(15/15%) 0.86 89.1 - - [42]
Soybean peroxidase Soybean PEG4000–IDA–Cu2+/Na SO (4/ 10%) 0.05 64 - - [75]
G6PDH Sigma (USA) PEG)/phosphate buffer(17.5/ 15%) 351 97.7 - 7.5 [58]
β-gala.; β –gluc. Barley PEG1500/ (NH4)2SO4 (14/ 13%) 2.7; 2.8 98.26; 92.58 19.65 6.9; 6.5 [56]
Lipase (E.C. 3.1.1.3) Burkholderia pseudomallei 2-propanol/phosphate (ATPS) (16/ 4.5%) 13.5 99 - 9.0 [40]
PPL Sigma  (USA) PEG1500/potassium  phosphate (17/13%) 12.7 94.7 - 7.0 [14]
PheDH Bacillus sphaericus PEG-6000/(NH4)2SO4 (8.5/17.5%) 0.027 94.42 39.89 8.0 [59]
Invertase Baker’s  yeast PEG3000/MgSO4(15/123%)+5%MnCl2 - 98 - 5.5 [57]
Plant-esterase Wheat flour PEG1000/NaH2PO4(27.0/13.0%) and PEG1000/NaH2PO4/(NH4)2SO4 (27.0/13.0/6.0%) - 83.16 - 5.0 [65]
Kp = partition coefficient of protein, TLL= Tie line length, IDA= Iminodiacetic acid, G6PDH= Glucose-6-phosphate dehydrogenase, pk= purification factors, β-gala =β-galactosidase, β–glu =β–glucosidase, PheDH= phenylalanine dehydrogenase, PPL= porcine pancreatic lipase, (-) = the value was not given
Table 2: Optimizing partitioning of different enzymes recovered in the various ATPS.