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Figure 3A: Comparison of the DNA recovered using P-QG2, P-W1, generic brands A and B’s PCR purification buffers. Bar chart showing the means and standard errors of the DNA recovered using various buffers. P-QG2-A and P-W1-A buffer testing were carried out using brand A’s protocol (see Supplementary Material). On the other hand, P-QG2-B and P-W1-B buffers were performed on brand B’s spin column (without P-BK). DNA concentrations were determined by IMPLEN Nanophotometer P330 in three separate extractions measured in triplicates. Gen A = PCR Purification using generic A PCR Purification Kit; P-QG2-A = PCR Purification using generic A column with P-QG2 buffer; P-W1-A = PCR Purification using generic A column with P-W1 buffer; Gen B = PCR Purification using generic B PCR Purification Kit; P-QG2-B = PCR Purification using generic B column with QG2 buffer; P-W1-B = PCR Purification using generic B column with P-W1 buffer. *p < 0.05; **p < 0.001 for the ANOVA tests comparing the DNA recovered. |