Run No. Block A: Post-Feed Glucose concentration (g/L) B: Seeding Density (x 106cells /mL) C: Temperature (°C) D: Dissolved Oxygen (DO)
1 1 8 0.3 35 60
2 1 5 0.4 36.5 45
3 1 8 0.5 35 30
4 2 8 0.3 38 30
5 2 2 0.3 38 60
6 2 5 0.4 36.5 45
7 3 2 0.3 35 30
8 3 2 0.5 35 60
9 3 8 0.5 35 60
10 4 5 0.4 36.5 45
11 4 5 0.4 36.5 45
12 4 2 0.5 38 30
Table 2: Half-fractional factorial design for the validation of process control parameters for fed batch.