Tree Part Drying method, Solvent Compounds Identification Ref. species extraction         Scots pine heartwood Oven-dried 60˚C, 48h,80% acetone Total phenolics 6.7-13.6 mg TAEg-1 Folin-Ciocalteau [26] heartwood Oven-dried 60˚C 48h,acetone Total pinosylvins 4.7-7.5 mg g-1Pinosylvin, PMME, PDME GLC-MS [26] heartwood Oven-dried 60˚C 48h,80% acetone Total phenolics 4.55-4.66 mg TAEcm-1 Folin-Ciocalteu [27] heartwood Oven-dried 60˚C 48h,acetone Pinosylvin, PMMEand PDME 2.07-3.16 mg cm-1 GLC-MS [27] knotwood Freeze-dried 1. hexane 2. acetone:water pinosylvin, PMME, DHPMME chromatographic purification, chrystallization GC after [28] knotwood Freeze-dried, 1.Hexane 2.Acetone Nortrachelogenin fractionated by flash chromatography GC-MS [29,30] knotwood N.a., 1.Hexane 2.Acetone/water (95:5) Lingnans, stilbenes and flavonoids GC-FID [30] knotwood N.a., 1. hexane 2. acetone Pinosylvin, PSME Fractionationby flash chromatography GC-MS [30] knotwood,bark Freeze-dried, 1.Hexane 2.Acetone Pinosylvin Fractionation using flashchromatography GCFID HPSEC [31] knotwood Resin Methyl-tert-butyl ether. Pinoresinol Purification with flashchromatography GC-FID [31] HPSEC       phloem Freeze/vacuum dried,80% methanol     [32] phloem,bark Air-dried 1.70% acetone 2.Chloroform 22 phenolic compounds HPLC-DAD HPLC-ESI-MS [33] needle, cork,bark Air dried,80% methanol Phenolics Folin-Ciocalteau [34] needles Distillation Essential oil (mainly terpenes and terpenoids) GC [35] Norwayspruce knotwoodbark 1.Hexane 2.Acetone/water (95:5) Lingnans, stilbenes and flavonoids GC-FID [30] knotwood Freeze-dried, 1.Hexane 2.Acetone/water (95:5) Hydroxymatairesinol GC-MS [30] sapwood,bark Methanol Isorhapontin, astringin, piceid,piceatannol, catechin, epicatechin HPLC [36] phloem Freeze-dried,80% ethanol Isorhapontin, astringin, piceid GC-MS [37] phloem Freeze-dried,80% methanol Isorhapontin, astringin, piceid,taxifolingulcoside, (+)-catechin HPLC [38] Silver birch bark 1.Hexane 2.Acetone/water (95:5) Lingnans, stilbenes and flavonoids GC-FID [30] phloem,bark Air dried,80% methanol Phenolics Folin-Ciocalteau [34] ASE = accelerated solvent extractor, DHPMME = dihydropinosylvin monomethyl ether, FID = flame ionization detector, PDME = pinosylvin dimethyl ether, PMME = pinosylvin monomethyl ether, TAE = tannic acid equivalent

Table 1: Extraction and identification methods of antibacterial substances.