Figure 4: SDS-PAGE analysis was performed to check the purity of purified C1-INH protein in reference to C1-INH standard (R&D systems). Samples were prepared in loading buffer containing 60 mM Tris-Cl pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol, 0.01% bromophenol blue and run on a 10% polyacrylamide gel. Lane 1: Purified C1-INH, 10 μg, +βME; Lane 2: Reference standard 15 μg, +βME; Lane 3: Purified C1-INH, 15 μg, +βME; Lane 4: Molecular weight marker, 29-205 KDa; Lane 5: Purified C1-INH, 15 μg,- βME; Lane 6: Reference standard 15 μg,-βME; Lane 7: Purified C1-INH, 10 μg,-βME.