Substrate Ratio of substrate (w/w) EI (oil) (%) EI( diesel) (%) ODA(cm) DCA Grease waste + wheat bran 1:1 43 ± 0.12 22 ± 0.01 3 ± 0.15 ++ waste cooking oil+ wheat bran 1:1 40 ± 0.15 20 ± 0.05 5 ± 0.90 ++ soybeans oil+ wheat bran 1:1 53 ± 0.20 37 ± 0.04 5 ± 0.05 +++ oil cake+ wheat bran 1:1 25 ± 0.62 10 ± 0.90 2 ± 0.01 +

Table 1: Influence of substrate sources for biosurfactant production in terms of EI: Emulsification Index; ODA: Oil Displacement Assay; DCA: Drop Collapse Assay; (+: less dispersed drop; ++: moderate dispersed drop; +++ highly dispersed drop of surfactant).