pH range EI (oil) (%) EI (diesel) (%) ODA (cm) DCA
3 37 ± 0.09 14 ± 0.28 2.2cm +
4 40 ± 0.05 19 ± 0.04 3.3 cm +
5 4 ± 0.30 22 ± 0.07 3.9cm ++
6 45 ± 0.03 23 ± 0.80 4.1m ++
7 45 ± 0.09 23 ± 0.9 4.8cm +++
8 47 ± 0.61 38 ± 0.09 5.5 +++
9 45 ± 0.07 30 ± 0.45 5.3 +++
10 44 ± 0.10 25 ± 0.03 4.7 ++
11 43 ± 0.01 18 ± 0.18 4.1 ++
12 38 ± 0.09 15 ± 0.03 3.4 +
Table 4: Influence of different pH values for production of biosurfactant in terms of EI: Emulsification Index; ODA: Oil Displacement Assay; DCA: Drop Collapse Assay; (+: less dispersed drop; ++: moderate dispersed drop; +++ highly dispersed drop of surfactant).