Medium components Coded values
-1.68 -1 0 1 1.68
  Caffeine concentration X1(g/l)   0.32   1   2   2   3.68
Initial cell loading X2(g/l) 1.27 4 8 12 14.73
pH X3 6.32 7 8 9 9.68
Table 1: Coded and actual values of the medium components chosen for optimization using CCD.