Samples Protein (g/L) β-glucan (pg/mL) β-glucan (pg/mg) Log10reduction Lot 1 Lot 2 Lot 1 Lot 2 Lot 1 Lot 2 Lot 1 Lot 2 MFP 0.76 1.07 8,847 15,181 11,580 14,188 - - QPAP 8.30 10.98 1,335 4,878 161 444 1.9 1.5 MMC 5.36 7.78 330 172 62 22 0.4 1.3 AEX 4.84 7.72 128 167 26 22 0.4 0.0 UF 85.1 81.8 856 1,588 10 19 0.4 0.1

Table 1: β-glucan concentrations measured in P. pastoris produced mAb A process-intermediates and the log10 reduction from each purification step.