Samples Protein (g/L) β-glucan (pg/mL) β-glucan (pg/mg) Log10reduction mAb B mAb C mAb B mAb C mAb B mAb C mAb B mAb C HCCF 2.3 2.7 73,928 152,059 32,143 56,318 - - NVIP 8.9 17.5 38.2 79.6 4.3 4.6 3.9 4.1 AEX 8.2 8.9 70.7 44.6 8.6 5.0 - 0.3 - 0.0 VF 7.8 5.4 80.7 626 10.3 116.0 - 0.1 - 1.4 UFDF 66.4 50.2 517 3,974 7.8 79.2 0.1 0.2

Table 2: β-glucan concentrations measured in CHO-produced mAb B and mAb C process-intermediates and the log10 reduction from each purification step.