Figure 4: Mature radial glia from primary neuroglial cultures of S. aurata brain. Phase contrast microscopy images showing radial glia progeny from neural stem cells. (a, b) Rosette-like colonies of radial glia after 2 weeks of culture (200x). (c) Edge colony enlargement of (a); detail of the radial glial fibers, making an interlaced scaffold to which NPCs were closely apposed, showing the nuclei migration of new neurons (red dashed circles) within the radial migrating fiber towards the boundary (400x). (d) Immunofluorescence staining focusing GFAP positive astrocytes (red) differentiated into large cells with many filopodia while contacting multiple axons (green filament) in order to sustain oligodendrocytes during the myelination process (green spheres) (GFAP, red; vimentin, green filament; MBP, green spheres; DAPI/PCNA, blue/cyan). Merged picture (e) displaying colocalisation of MBP to label OPCs (green spheres) and GFAP to label astrocytes (red) near NF positive axons (green), with DAPI/PCNA (blue/cyan) to label cell nuclei. (d, e) RGCs can produce new generation of young neurons even in the old culture as revealed by PCNA within the inner cluster. (f) This diagram shows a schematic arrangement of nervous cells in culture. Astrocytes contact neurons being involved in metabolic exchange between neurons and blood; oligodendrocytes wrap themselves around axons forming the myelin sheath. Scale bar = 50 μm (a, b, d, e); 10 μm (c).