Time (min) Percent Dye decolourization at varying temperatures at
30°C 40°C 50°C
DR19 495nm) DR19+ DB9 (λ460nm) DR19 495nm) DR19+ DB9 (λ460nm) DR19 495nm) DR19+ DB9       (λ460nm)
S- PGP I-DEAE S-PGP I-DEAE S- PGP I-DEAE S-PGP I-DEAE S- PGP I-DEAE S-PGP I-DEAE
20 70.8 72.5 59.2 62.5 74.8 76.5 66.2 70.5 61.8 62.5 46.2 61.5
40 70.6 72.6 58.7 63.6 74.6 78.4 67.7 72.6 61.6 62.6 48.7 62.6
60 72.8 74.4 57.4 65.5 78.8 81.3 69.4 73.5 60.8 64.4 49.4 65.5
80 73.6 76.4 54.5 65.5 79.9 86.4 71.5 75.5 60.6 66.4 53.5 67.5
100 73.7 76.4 54.6 65.4 79.9 86.9 72.4 77.4 58.7 66.4 55.6 67.9
120 76.9 78.4 55.1 65.4 80.9 88.2 74.1 77.4 55.9 58.4 51.2 63.4
140 60.4 72.2 33.2 64.3 65.4 88.1 45.2 69.3 47.4 58.2 36.2 63.3
160 50.3 69.1 30.4 60.2 52.7 88.3 39.8 69.1 31.3 48.1 29.4 60.2
Table 3: Dye decolourization in batch processes. DR 19 or mixture of dyes (DR19+DB9) (200 mL) was independently treated with soluble and immobilized PGP (180 U) for varying times and temperatures in batch processes. Aliquots were taken from containers at different time intervals for measuring the dye colour removal. Each value represents the mean for three independent experiments.