Theoretical Model NaCl concentration (mmol L−1)
200 600 800 1000
Langmuir        
Kd (µmol L−1) 0.2751 0.6374 0.8472 1.1984
qm(mg/mg gel) 274.40 264.55 263.59 241.61
Freundlich        
K (10-3L/mg gel) 176.2 150.7 135.7 116.7
η 2.6207 2.0082 1.8562 1.6259
Temkin        
KT(L moL−1) 22.5191 6.6284 4.5021 2.594
qT(mg/mg gel) 57.9874 77.4595 83.3876 95.5819
Langmuir-Freundlich        
K*d(mM) 0.318 0.4502 0.3872 0.2785
qm(mg/g gel) 242.2 244.7 237.8 178.5
η 1.85 1.16 1.12 0.99
a Buffer composition: HEPES 20mmol L−1; 200-1000mmol L−1 NaCl; pH 8.0; 30°C.
Table 1: Estimated parameters of isotherm models for adsorption of whey lactoferrin on IDA-Cu2+- cryogel system at different ionic strength conditionsa.