Figure 5: qPCR analysis of the effect of ICSD on advanced dopamine neuron gene expression over the first 11 days of neuronal priming. Day 5 and day 11 columns represent dopamine neuron associated gene expression relative to day 0 expressions at the different ICSD. TH expression in hESC was observed to significantly increase over the course of the protocol; this was not observed in hiPSC. In both cell lines lmx1a expression was shown to either be stable or decrease over the course of the protocol. (*p< 0.05 & **p<0.01, Bars represent Mean ± SEM, n=3)