Figure 6: HPLC analysis results of the substrate cellobiose and product glucose by 1,4- β-D-glucan glucohydrolase with the time course of the reaction. HPLC (Younglin Instrument Acme 9000, Korea) analysis was conducted under conditions of Shodex Asahipak column NH2P-50 4E, fluent of water/CH3CN (1:3), flow rate of 1 ml/min., detector of RI (refractive index), column temperature of 30°C.