Purification step
Total Vol. (ml)
Total Activity(U)
Total Protein (mg)
Specific activity (U/mg)
Fold Purification
Yield (%)
Crude Sample
200
3000
260
11.5
1
100
Ammonium sulfate Precipitation
25
2160
102
21.2
1.84
72
Gel filtration
10
1257
21
59.9
5.17
42
Ion Exchange Chromatography
10
740
7
105.5
9.7
25
Table 4:
Summary of purification of ALP.
