Run Buffer pH Concentration of  buffer salt (mM 1AS 2AS 1NaCl 2NaCl
1 2AS+Citric acid/sodium citrate 50M 5 50 68.36 778.87 5.66 18.39
2 2AS+ Citric acid/sodium citrate 100M 5 100 39.76 1061.93 16.95 45.28
3 2AS+ Citric acid/sodium citrate 150M 5 150 119.38 966.63 4.56 85.98
4 2AS+50m TrisHCl 7 50 42.89 1875.58 85.09 205.51
5 2AS+100M TrisHCl 7 100 352.22 1174.42 2.12 79.92
6 2AS+150M TrisHCl 7 150 576.46 906.18 18.27 117.52
7 2AS+50M TrisHCl 9 50 156.36 674.13 23.58 86.23
8 2AS+100M TrisHCl 9 100 500.85 959.43 356 9.89
9 2AS+150M TrisHCl 9 150 57.62 1210.68 243 26.99
Table 2: Area under the peak value at different buffer concentration.