Culture media     viable apoptotic necrotic viable apoptotic necrotic Negativecontrol Glass 97.0% 1.9% 1.0%           After 20 min at RT After 80 min at RT Thermally treated Glass 86.2% 6.6% 7.2% − − − PNIPAM 88.1% (±2.3%) 5.6% (±0.9%) 6.1% (±3.3) 24.5% (±6.9%) 12.8% (±3.5%) 61.9% (±4.7%)

Table 1: Cell viability versus thermal treatment duration. 1000 events were considered for each condition for FACS analysis.