Sample
Activity (U/ml)
Protein content (mg/ml)
Specific activity (U/mg)
Purification fold
Crude fermented Extract
1160
2.6
446
1
30-80% (NH
4
)
2
SO
4
saturation
6820
4.1
1664
3.7
DEAE cellulose chromatography (Flow through)
4259
1.6
2662
6
Table 2:
Partial Purification of amylase from Bacillus subtilis (ATCC 6633) fermented extract .