Independent variable   Coefficient Symbol Soluble proteins(1) (Y1) NH2 –free amino groups (2) (Y2)
Estimate t-value P-value % confidence Estimate t-value P-value % confidence
B0 0.1095 24.1899 6.99E-05* 99.99 45.7075 19.1920 0.000327* 99.97
X1: Feather % (W/V) B1 0.0265 5.85419 0.00274* 99.726 12.1592 5.10548 0.015578* 98.44
X2:Peptone % (W/V) B2 0.0005 0.11045 0.118777   -0.9925 -0.416 0.807205  
X3:Yeast extract % (W/V) B3 0.0190 4.19734 0.006028* 99.3972 5.61083 2.35592 0.106506  
X4:NH4Cl % (W/V) B4 -0.02267 -5.0073 0.002601* 98.9103 9.33083 3.917901 0.030684  
X5:Time (hrs) B5 0.007333 1.62002 0.010897 98.9103 4.83417 2.02981 0.137648  
X6:Inoculum size % (V/V) B6 0.052333 11.5611 0.001033* 99.8967 1.4641 0.61479 0.575616  
X8:Casamino acids % (W/V) B7 0.0060 1.32547 0.978258   -6.00083 -2.51968 0.088292  
X8:K2HPO4 % (W/V) B8 -0.01067 -2.356 0.898196   -1.40583 -0.59029 0.617889  
• Highlighted significant model terms are taken at P ≤ 0.006 for the soluble protein model. • Highlighted significant model terms are taken at P ≤ 0.02 for the NH2-free amino groups model. (1): R2=0.99 and adjusted R2=0.97 (2): R2=0.95 and adjusted R2=0.85
Table 4: Regression summary for full polynomial equation for end products of feather biodegradation upon using PBD.