Figure 5: Effects of pH after (NH4)2SO4 precipitation, (NH4)2SO4 concentration and VCR of the first UF, respectively, on both β-amylase recovery and the specific enzyme activity in the supernatant obtained following the second lowspeed centrifugation (i.e. the feed to the second UF). (▼) recovery of β-amylase activity in this supernatant; (○) β-amylase specific activity (U/mg protein) in the supernatant. (A) The effect of VCR of the first UF, when the (NH4)2SO4 concentration was kept at 1.2 M and pH adjusted to 4 before the second UF.(B) The effect of (NH4)2SO4 concentration, when the first UF terminated at VCR 7 and pH adjusted to 4 before the second UF.(C) The effects of pH after (NH4)2SO4 precipitation, when the first UF terminated at VCR 7 and (NH4)2SO4 at 1.2 M.