| Sample ID |
Description of process steps |
Exemplified volume (L) |
β-amylase activity (U/ml) |
Specific enzyme activity (U/mg protein) |
Accumulated enzyme recovery (%) |
Purification factor |
| Sample 1# |
Soybean whey “wastewater” (pH 4.5, temperature 45°C, β-amylase biological activity 11 U/ml, protein 0.38 (w/w)%, total sugar 1.27%, ash content 12.5%, total solid 2.5%. |
20 |
11 |
2.9 |
100 |
1.0 |
| Sample 2# |
Sample 1# adjusted to pH 7 and following centrifugation the supernatant filtered by 3 kDa MWCO UF to obtain retentate. |
2 |
101 |
3.5 |
92 |
1.2 |
| Sample 3# |
Sample 2# salting-out using (NH4)2SO4, pH adjusted to 4. The obtained centrifugal supernatant is pH further adjusted to 7, followed by 30 kDa MWCO UF to obtain retentate. |
0.24 |
674 |
14 |
74 |
4.8 |
