S.No Variable Code Levels
      -2 (-α) -1 0 1 2 (+α)
1. Temperature (°C) A 18.18 25.00 35.00 45.00 51.82
2. Incubation time (h) B -10.99 4.00 26.00 48.00 62.99
3. Hypochlorite concentration (%) C -3.64 10.00 30.00 50.00 63.64
Table 2: Levels of physical factors used for optimization of three factors affecting PHB recovery