Run Order Run Levels Levels Temperature (°C) Hypochlorite Concentration (% v/v) Incubation period (h) PHB recovery (%)
17 1 0 1 35.0000 30.0000 26.0000 94.0 ± 0.25
19 2 0 1 35.0000 30.0000 26.0000 95.0 ± 0.21
20 3 0 1 35.0000 30.0000 26.0000 96.0 ± 0.35
5 4 1 1 25.0000 10.0000 48.0000 83.0 ± 0.4
6 5 1 1 45.0000 10.0000 48.0000 88.0 ± 0.15
10 6 -1 1 51.8179 30.0000 26.0000 93.0 ±± 0.38
3 7 1 1 25.0000 50.0000 4.0000 78.0 ± 0.2
8 8 1 1 45.0000 50.0000 48.0000 86.0 ± 0.35
7 9 1 1 25.0000 50.0000 48.0000 89.0 ± 0.26
9 10 -1 1 18.1821 30.0000 26.0000 90.0 ± 0.25
11 11 -1 1 35.0000 -3.6359 26.0000 78.0 ± 0.50
4 12 1 1 45.0000 50.0000 4.0000 85.0 ± 0.42
15 13 0 1 35.0000 30.0000 26.0000 95.0 ± 0.38
12 14 -1 1 35.0000 63.6359 26.0000 82.0 ± 0.53
14 15 -1 1 35.0000 30.0000 62.9994 85.0 ± 0.53
13 16 -1 1 35.0000 30.0000 -10.9994 79.0 ± 0.3
16 17 0 1 35.0000 30.0000 26.0000 95.5 ± 0.21
2 18 1 1 45.0000 10.0000 4.0000 88.0 ± 0.25
18 19 0 1 35.0000 30.0000 26.0000 94.0 ± 0.25
1 20 1 1 25.0000 10.0000 4.0000 86.0 ± 0.40
17 1 0 1 35.0000 30.0000 26.0000 95.0 ± 0.32
Table 3: Central composite design of independent variables for the optimization of parameters affecting PHB recovery from Alcaligenes sp.