Figure 1: Estimation of protein-potentially-contributed-by-cell-death (PPCD) in culture medium. For this, CHO-K1 cells were grown in suspension culture in CD-CHO media (serum-free and chemically defined) and cell counts were performed everyday using trypan-blue dye exclusion method. Pre-decided amount of culture media was collected and centrifuged at 1000 rpm at 4°C for 20 min. The supernatant was concentrated to known volume using 5 kDa molecular weight cut-off centrifugation filters to enable the calculation of protein content per milliliter (mL) in original sample. Known number of cells was sampled and lysed using urea-based lysis buffer. Protein concentration was estimated in concentrated media and cell-lysate using Bradford protein estimation method. Per-cell-protein content was calculated by dividing the total amount of protein with total number of cells in the sample. The concentration of PPCD was calculated by multiplying the number of dead cells/mL with per-cell-protein content in the sample at that respective time-point. A: cell growth; B: per-cell-protein content, C: protein concentration detected in spent-media and D: concentration of PPCD. Error bars represents standard deviation among three biological replicates.