Fatty acid Percentage of fatty acids in different cultures (%) Control PP BP CW PM MO CD SU GL BH CY C8:0 15.2 2.9 3.6 12.7 5.1 7.6 3.7 9.4 14.2 7.1 5.7 C10:0 0.2 - 0.9 - - - - 0.6 - 1.1 1.4 C11:0 15.1 3.9 5.4 13.5 7.1 9.4 4.8 11.4 16.1 9.4 7.8 C12:0 - 0.6 2.1 - 1.9 - - 1.4 - 1.5 2.1 C13:0 13.9 6.2 5.4 15.4 10.4 11.9 5.4 12.2 14.8 11.1 9.3 C14:0 - - - - - - - - - 1.2 - C14:1 - - - - - 1.4 - - 11.2 1.1 1.1 C15:0 11.6 5.1 3.8 13.7 11.0 11.3 4.3 8.1 - 11.2 6.5 C15:1 - - 2.1 - - - - - 7.5 - - C16:0 10.7 55.9 56.7 12.4 4.1 5.5 60.1 8.5 9.6 12.8 25.7 C16:1 3.6 4.8 2.5 - 5.8 8.7 2.9 9.3 - - 6.2 C17:0 - - - 1.9 - - - - - 4.8 - C17:1 - - - - - - - - 0.8 - - C18:0 3.2 2.1 2.2 1.2 5.8 2.3 1.1 1.2 - - 1.9 C18:1 - 1.7 - 0.5 5.4 4.1 3.2 8.6 6.2 4.3 2.8 C18:2 8.3 3.6 2.0 6.2 8.5 3.1 1.2 1.3 - - 2.3 C20:0 - 0.9 - 1.8 2.3 - - - 0.8 - 2.1 C18:3 - - 1.8 0.2 3.9 9.3 2.5 6.3 - 7.5 4.4 C20:1 - - 1.3 - 0.7 - - - 4.9 - - C21:0 6.8 1.8 - 4.5 3.2 2.1 1.4 2.6 - 3.1 2.3 C20:2 - - - - - - - - 0.6 - - C22:0 - - - 0.6 0.9 - - - - - - C22:1 - - - - - - - - - - 6.2 C20:3 1.5 2.2 - 2.5 6.7 8.2 1.2 6.9 3.14 5.6 1.5 C23:0 4.2 1.2 1.6 2.6 2.6 2.1 0.8 2.1 - 1.1 - C22:2 - - - 0.6 1.1 - - - - 1.4 - C24:0 - - - 2.1 1.2 1.7 - - - - - C20:5 - - 0.6 0.6 1.0 - - 1.4 2.1 - 1.2 C22:5 - - - 1.3 - - - - - - - C22:6 6.7 - - 0.7 0.8 0.7 0.4 0.3 0.7 1.1 0.7

Table 4: Fatty acid composition of control and waste nutrient supplemented cultures of C. reinhardtii.