S No. Accession Description Gene Symbol Score Coverage Peptides AAs MW [kDa] calc. pI
  Structural
1 347360906 Actin, cytoplasmic 1 ACTB 149.59 17.6 6 375 41.7 5.39
2 354492323 Moesin MSN 54.27 3 2 566 66.5 6.28
3 354482483 Vimentin VIM 283.18 14.16 8 466 53.7 5.12
4 354503280 Annexin A2-like, partial ANXA2 220.87 39.65 8 227 25.4 5.97
5 354501786 Cofilin-1-like CFL1 106.65 30.72 3 166 18.5 8.09
6 354489212 Myosin-Ic-like isoform 1 MYO1C 163.56 7.49 5 1028 118.1 9.38
7 345842430 Tubulin alpha-1B chain TUBA1B 129.5 12.64 4 451 50.1 5.06
8 354472141 Alpha-actinin-1 ACTN1 79.43 2.58 2 931 107.5 5.39
9 354504022 Myosin-9 MYH9 434.54 7.5 11 1880 217.6 5.64
10 354496812 Annexin A1-like ANXA1 202.12 21.1 6 346 38.8 7.02
11 354482221 Annexin A11 ANXA11 81.65 4.57 2 503 54.1 7.65
12 354491741 Annexin A4-like isoform 1 ANXA4 78.41 13.17 3 319 35.8 5.57
13 354497282 Actin, cytoplasmic 2-like, partial ACTG1 149.59 18.97 6 348 38.5 5.36
14 354490960 Ezrin EZR 437.42 14.89 9 638 74.9 6.89
15 354481554 Prelamin-A/C-like isoform 1 LMNA 143.58 6.63 4 664 73.9 7.81
16 354481232 Radixin RDX 54.27 2.92 2 583 68.5 6.27
17 354483018 Basement membrane-specific heparan sulfate proteoglycan core protein HSPG2 173.12 2.06 7 4412 471.6 6.54
18 354473830 Tropomyosin alpha-1 chain-like TPM1 377.6 35.21 10 284 32.8 4.75
19 354478924 Tropomyosin alpha-3 chain-like isoform 3 TPM3 405.59 35.08 10 248 28.9 4.75
20 354500481 Filamin-A FLNA 448.02 8.83 13 2538 268.8 5.9
21 354481182 Filamin-B isoform 2 FLNB 168.01 2.73 5 2603 277.7 5.74
22 354494381 Fibronectin isoform 1 FN1 183.15 3.59 7 2480 272.7 5.63
23 354472895 Histone H4-like HIST1H4A 80.76 29.13 3 103 11.4 11.36
24 354483672 Spectrin beta chain, brain 1 isoform 1 SPTBN1 137.69 2.37 4 2363 274 5.59
25 354501005 Lactadherin-like, partial MFGE8 195.72 37.93 5 174 19.9 8.65
  Metabolism
1 354496013 Fatty acid-binding protein, adipocyte-like FABP4 124.41 24.24 4 132 14.7 7.93
2 354473486 Pyruvate kinase isozymes M1/M2-like isoform 1 PKM 182.09 17.14 6 531 57.9 7.27
3 354499945 Alpha-enolase ENO1 245.84 16.78 5 429 46.7 6.16
4 349501082 Glyceraldehyde-3-phosphate dehydrogenase GAPDH 171.5 13.51 3 333 35.7 8.34
5 354474551 Acyl-CoA-binding protein-like DBI 190.54 28.74 3 87 10 8.82
6 354472325 Tubulointerstitial nephritis antigen TINAGL1 53.31 5.59 2 465 52.5 7.08
7 354476904 Sodium/potassium-transporting ATPase subunit alpha-1 ATP1A1 134.37 5.65 4 1116 123 5.53
8 354468711 Cathepsin Z CTSZ 118.16 13.4 3 306 34 7.58
9 354478439 Nucleoside diphosphate kinase B-like NME2 162.48 35.53 5 152 17.3 7.99
10 350537635 4F2 cell-surface antigen heavy chain SLC3A2 142.27 15.01 4 533 58.9 5.47
11 346227178 L-lactate dehydrogenase A chain LDHA 124.31 14.16 4 332 36.5 7.42
12 354472752 Cytochrome c, somatic-like CYCS 71.43 20.95 2 105 11.7 9.51
13 354482641 Delta-aminolevulinic acid dehydratase ALAD 183.01 17.58 5 330 36.1 6.98
14 354497685 Pantetheinase VNN1 73.08 5.47 2 512 56.7 5.63
  Cell Signaling
1 354469007 Rho GDP-dissociation inhibitor 1-like ARHGDIA 237.74 31.86 4 204 23.4 5.2
2 354496917 Calmodulin-like CALM1 984.41 22.26 6 265 29.1 4.75
3 354466994 Phosphatidylethanolamine-binding protein 1-like PEBP1 214.21 49.2 5 187 20.9 7.53
4 354478483 Caltractin-like CETN2 984.41 18.79 6 314 34.8 5.82
5 354478978 Protein S100-A6-like S100A6 467.41 28.09 2 89 10 5.48
6 354484391 14-3-3 protein zeta/delta-like YWHAZ 165.18 22.86 4 245 27.7 4.79
7 354476375 Transforming protein RhoA-like RHOA 65.91 13.47 2 193 21.8 6.1
8 354501872 Rho-related GTP-binding protein RhoC-like RHOB 65.91 13.47 2 193 22 6.58
9 354487034 Macrophage-capping protein-like CAPG 111.01 10.03 2 349 38.7 6.73
10 354471813 EH domain-containing protein 4, partial EHD4 57.66 3.69 2 515 58.6 6.58
11 350539723 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 GNB1 166.79 13.82 4 340 37.4 6
12 354508126 14-3-3 protein epsilon-like, partial YWHAE 103.43 33.33 3 105 11.9 5.83
13 354494229 SH3 domain-binding glutamic acid-rich-like protein-like SH3BGRL 144.62 74.56 5 114 12.7 5.07
14 354495613 Thrombomodulin-like THBD 68.15 6.24 2 577 61.9 4.69
15 354482553 Thrombospondin-1 THBS1 268.51 8.89 8 1170 129.6 4.93
  Transcription
1 354475571 NSFL1 cofactor p47-like isoform 1 NSFL1C 105.33 12.1 3 372 41 5.2
2 537153542 Bromodomain adjacent to zinc finger domain protein 1A BAZ1A 89.58 2.39 3 2011 228.4 6.09
3 537191106 Nuclear factor related to kappa-B-binding protein NFRKB 34.07 0.6 1 2505 275.9 7.68
4 354502621 Nuclease-sensitive element-binding protein 1-like YBX1 231.37 21.36 4 337 38.3 10.15
  Translation
1 346227155 Elongation factor 2 EEF2 110.3 3.61 3 858 95.3 6.83
2 354486063 Clusterin-like CLU 67.54 7.42 2 445 51.5 5.85
3 346986359 Elongation factor 1-alpha 1 EEF1A1 95.09 8.44 2 462 50.1 9.01
4 354495391 60S acidic ribosomal protein P2-like RPLP2 60.2 24.35 2 115 11.7 4.54
  Proteosomal Degradation Complex
1 350540096 Lysosome-associated membrane glycoprotein 1 LAMP1 146.08 9.34 3 407 43.8 6.71
2 354494438 Sulfated glycoprotein 1 PSAP 126.27 7.53 3 598 65.7 5.49
3 354468731 Proteasome subunit alpha type-7-like PSMA7 112.06 7.59 2 382 41.7 9.61
4 346986300 Polyubiquitin UBB 64.28 31.31 2 1038 116.6 8.38
5 354473967 Ubiquitin-60S ribosomal protein L40-like isoform 1 UBA52 64.28 19.53 2 128 14.7 9.83
6 354483686 Ubiquitin-40S ribosomal protein S27a-like RPS27A 64.28 16.03 2 156 17.9 9.6
7 537136494 Ubiquitin carboxyl-terminal hydrolase 5 isoform 2 USP5 41.67 2.47 2 1051 116.4 5.12
  Stress Response
1 354466320 Superoxide dismutase [Cu-Zn]-like SOD1 114.63 14.88 2 242 25.9 9.55
2 350537945 Peroxiredoxin-1 PRDX1 60.01 12.56 3 199 22.2 8.05
3 354487462 Thioredoxinreductase 1, cytoplasmic TXNRD1 431.06 24.88 10 611 67.2 7.8
4 354470385 Catalase CAT 121.73 6.83 3 527 60 7.68
5 354507545 Glutathione S-transferase Mu 1-like GSTM1 76.36 10.09 2 218 25.5 6.37
6 354506476 Glutathione S-transferase Mu 7-like GSTM7 76.36 10.09 2 218 25.9 7.37
7 350537543 Glutathione S-transferase P 1 GSTP1 107.27 12.38 2 210 23.6 7.8
  Cell adhesion
1 354496408 Galectin-1-like LGALS1 67.81 24.44 2 135 14.8 5.8
2 350539683 Intercellular adhesion molecule 1 ICAM1 119.38 9.3 4 527 57.6 7.24
3 354473282 Galectin-3-binding protein-like LGALS3BP 130.62 10.8 4 574 63.8 5.19
4 537201836 Hyaluronan and proteoglycan link protein 3 HAPLN3 356.88 18.05 13 881 97.9 7.62
  Protein folding (molecular chaperones)
1 354483044 Peptidyl-prolylcis-trans isomerase A-like PPIA 145.54 22.56 3 164 17.9 8.28
2 354474350 Peptidyl-prolylcis-trans isomerase B-like PPIB 80.95 12.5 2 216 23.6 9.58
3 350537423 78 kDa glucose-regulated protein HSPA5 344.8 22.48 12 654 72.3 5.16
4 350539823 Heat shock cognate 71 kDa protein HSPA8 146.65 12.07 5 646 70.8 5.36
5 350540064 Heat shock protein HSP 90-alpha HSP90AA1 85.04 5.59 3 733 84.8 5.03
6 354479029 Heat shock protein HSP 90-beta-like HSP90AB1 140.59 7.04 4 724 83.1 5.02
  Miscellaneous
1 354480912 Basigin BSG 245.79 13.45 4 409 44.6 6.2
2 354501595 Complement regulatory protein Crry-like CD59 88.75 11.16 2 421 46.6 7.64
3 354485425 Platelet glycoprotein 4-like CD36 63.15 7.42 2 472 52.9 8.56
Table 3: List of identified unique proteins in microvesicles isolated from CHO batch culture.