Figure 4: Confocal microscopy images of samples taken from the cultivation of activated sludge (AS) under feeding of acetic acid-sodium acetate buffer system(treatment 4): (A) fluorescence image of the raw AS; (B) fluorescence image of the final lipid-enhanced AS; (C) overlay of fluorescence and transmitted light imagesof raw AS; (D)-(F) overlay of fluorescence and transmitted light images of final lipid-enhanced AS at varying magnification and location.