| Target gene
(product size) |
Primer sequence |
| Actc1 (124 bp) |
FW: 5’-CCA GAT CAT GTT TGA GAC CTT CAA-3’
RV: 5’-GAA CAT TAT GAG TTA CAC CAT CGC-3’ |
| BMP2 (249 bp) |
FW: 5’-GGG ACC CGC TGT CTT CTA GTG TTG C-3’
RV: 5’-TGA GTG CCT GCG GTA CAG ATC TAG CA-3’ |
| -Fetoprotein (173 bp) |
FW: 5’-TCG TAT TCC AAC AGG AGG-3’
RV: 5’-AGG CTT TTG CTT CAC CAG-3’ |
| Gata4 (207 bp) |
FW: 5’-CTG GAG GCG AGA TGG GAC GGG ACA CTA C-3’
RV: 5’-CCG CAG GCA TTA CAT ACA GGC TCA CC-3’ |
| GAPDH (150 bp) |
FW: 5’-CTA CCC CCA ATG TGT CCG TC-3’
RV: 5’-GCT GTT GAA GTC GCA GGA GAC-3’ |
| Nanog (163 bp) |
FW: 5’-GCG GCT CAC TTC CTT CTG ACT T-3’
RV: 5’-GAC CAG GAA GAC CCA CAC TCA T-3’ |
| Neurod3/ngn1 (405 bp) |
FW: 5’-CAT CTC TGA TCT CGA CTG C-3’
RV: 5’-CCA GAT GTA GTT GTA GGC G-3’ |
| Oct3/4 (459 bp) |
FW: 5’-CTG AGG GCC AGG CAG GAG GAG CAC GAG-3’
RV: 5’-CTG TAG GGA GGG CTT CGG GCA CTT-3’ |
| Zfp42/Rex1 (287 bp) |
FW: 5’-ACG AGT GGC AGT TTC TTC TTG GGA-3’
RV: 5’-TAT GAC TCA CTT CCA GGG GGC ACT-3’ |
