Figure 6: Curcumin alone and incorporated in micelles significantly increased human glioblastoma cell death. U251N cells were treated with curcumin alone(curcumin) or curcumin incorporated in micelles (curcumin micelles, (PEG750)2–PCL4700 ) for 24 or 20 hours with 0 or 4 hours of pifithrin-α (50 µM), for a total of 24 hours. Cells were then fixed and stained with the fluorescent Hoechst dye (10 µM, blue). Images were taken directly from the plate using Operetta imaging system (Perkin Elmer) and cell number was analyzed using the Harmony software. Viability of cells treated with curcumin alone (B) or curcumin in micelles (C) ((PEG750)2–PCL4700 or ((PEG750)2–PCL14200is expressed relative to untreated cells. Mean values ± SEM are calculated based on experiments ran in triplicates. Statistically significant differences from untreated control were calculated using a t-test of OriginPro software and indicated by * (p < 0.05) and ** (p < 0.01).