Run PLGA PVA Speed P1 100 0.5 5000 P2 100 3 5000 P3 300 3 20000 P4 300 0.5 5000 P5 300 0.5 20000 P6 100 3 20000 P7 300 3 5000 P8 100 0.5 20000

Factor Name Units Low Level High Level A PLGA mg 100 300 B PVA % 0.5 3 C Speed rpm 5000 20000

Table 1b: Experimental design of PTX-PLGA nanoparticles.