Figure 7: Splenic recall response of non-diabetic mice 20 weeks post BMDC treatment. A-E. 1x106 spleen cells from FBS+ BM-DC, FBS- BM-DC or PBS treated mice were re-stimulated with GAD217-236 peptide for 4 days. A. Tritiated thymidine uptake was measured for the final 16 hours of culture. B-E. Supernatant was removed after 72 hours of culture and the indicated cytokines were measured with a multiplex cytokine immunoassay.