Vacuum tube holder              Syringe and injection needle Syringe(20ml)
Vs.
n=171 Syringe(20ml) Syringe(20ml) Syringe(20ml) Vacuum Tube holder Syringe (10ml) Syringe (2.5ml)
n=191 n=39 n=39
Mean   SD Mean SD Mean SD Mean SD P-value
 Time from puncture to when movement stopped
Mean (seconds) 2.8
1.3
2.5
1.0
2.9
1.3
2.8
1.0

0.051†

0.038

0.091
Maximum (seconds) 8.8 7.3 8.0 5.5
Minimum (seconds) 0.0
0.7
0.0
1.3
Distance travelled by needle
Mean (mm) 12.4
4.0
12.1
4.5
14.9
4.7
15.4
3.8

0.586

0.001

P<0.001
Maximum (mm) 21.8 24.6 24.7 24.5
Minimum (mm) 0.2 0.8 0.4 9.3
Skin puncture Puncture angle

a)
Mean (°)  17.4
5.1
14.2
6.3
13.9
6.1
15.9
5.6

P<0.001

0.782

0.137
Maximum (°)  44.7 55.6 36.1 30.3
Minimum (°)  9.7 2.8 5.3 6.4

b)§
Mean (°)  14.4
4.9
8.6
6.8
7.7
3.5
10.3
7.2

P<0.001

0.376

0.156
Maximum (°)  30.8 55.3 14.4 44.9
Minimum (°)  0.2 0.3 0.4 1.0
 Angle changes (b-a) -3.0 -5.6 -6.3 -5.5
MP joint and puncture point
a) Mean (°) 36.1 8.6 28.9 10.4
30.7

9.4

32.8

10.2

P<0.001

0.311

0.033
Maximum (°) 65.9 77.1 57.2 65.2
Minimum (°) 17.4 5.4 11.7 18.2

b)§
Mean (°) 39.5
9.1
30.0 9.6 31.5
8.4
35.4
9.9

P<0.001

0.367

0.002
Maximum (°) 63.0 76.0 48.6 69.3
Minimum (°) 18.0 6.6 3.8 18.4
 Angle changes (b-a) 3.4 1.1 0.8 2.7 0.006
Differences in puncture points                                       n=166                      n=186
Mean (°)
Maximum (°)
Minimum (°)
3.9          3.1                  5.0          4.4
8.8          7.9                 21.4         6.2
0.4          0.4                 1.1           0.4
Student t: test p-value, : Welch’s t-test p-value, : Time point when the needle come into contact with the model §: The cessation of needle movement
Table 1: Differences in blood sampling using a 20 ml syringe, vacuum tube sampling, and a 10 ml or 2.5 ml syringe.