S.N. Intracellular Immunophenotypic expression subset  AML  (n=222) B-ALL n=176) T-ALL (n=44) MPAL B-My (n=14) T-My (n=2) 1 MPO-cCD3+cCD79a+/-Tdt+/- 0(0) 0 (0) 44 (100) 0 (0) 0 (0) 2 MPO-cCD79a+cCD3‑Tdt+/- 8(3.6) 174 (98.8) 0 (0) 0 (0) 0 (0) 3 MPO+cCD79a-cCD3-Tdt+/- 178 (80.1) 0 (0) 0 (0) 0 (0) 0 (0) 4 MPO+cCD79a+ cCD3-Tdt+/- 18(8.1) 0 (0) 0 (0) 14(100) 0 (0) 5 MPO-cCD79a-cCD3-Tdt+/- 18 (8.1) 2 (1.1) 0 (0) 0 (0) 0 (0) 6 MPO+ cCD79a-cCD3+Tdt+/- 0(0) 0(0) 0(0) 0(0) 2 (100)

Table 2: Different subset of cytoplasmic tubes with variable positive negative expression after ignoring Tdt status. Subset-1 had 100% sensitivity and 100% specificity for T-ALL diagnosis; Subset-2 had 98.8% sensitivity and 97.2 % specificity for B-ALL diagnosis; Subset-3 had 80.1% sensitivity and 100 % specificity for AML diagnosis; Subset-5 had 8.1% sensitivity and 99.1 specificity for AML diagnosis; Subset-3&5 in combination had 88.3% sensitivity and 99.1% specificity for AML diagnosis; Subset-4 had 100% sensitivity and 92.6% specificity for MPAL (BMy) diagnosis; Subset-6 had 100% sensitivity and 100% specificity for MPAL (T-My) diagnosis.