Figure 2: Adsorption/elution studies with the two-stage papain-IAT. (A) Adsorption studies were performed on RBCs with ccee (rr), DCCee (R1R1), and DCcEe (R1R2) phenotypes, and then the reactivity with RBCs with DCCee (R1R1), DccEE (R2R2), and ccee (rr) phenotypes was investigated. (B) The eluates of the adsorption studies were applied to GMA, and the reactivity with RBCs with DCCee (R1R1), DccEE (R2R2), DCcEe (R1R2), Ccee (r'r), ccEe (r''r), and ccee (rr) phenotypes is shown.