Figure 6: Anti-FVIII ELISA titer of rpFVIII (circles) and rpFVIIIND (squares). FVIII deficient mice were injected every 7 days with 2 μg/mouse recombinant porcine FVIII alone or porcine FVIII-ND complex. Each mouse received 4 doses, and plasma was obtained one week after the final dose. Serial dilutions (1:2) of plasma were added to ELISA plates coated with porcine or human FVIII, and the highest plasma dilution that produced a reading of ≥0.2 OD over background at 450 nm were reported as endpoint titers.