Samples Clotting time Activity Activity Diluted (sec) Diluted (U/ml) Total (U/ml) rpFVIII 58.1 0.19 1530 rpFVIII-ND 57.7 0.2 1777

Table 1: Activity of pFVIII and pFVIII bound to ND (pFVIII-ND) as measured with the one stage aPTT clotting assay in FVIII deficient human plasma. The pFVIII and ND were mixed in 10 times excess of ND to account that all pFVIII is bound to the ND. The ND alone didn’t have a procoagulant effect in FVIII deficient plasma.