Concentration in µg/ml
Mean area Response achieved
Response calculated thru Trend line equation
Residual (Response practical -Response theoretical)
Residual square
34.2
294426
288932.80
-5493.2
98899047.0
51.3
430896
436335.20
5439.2
1133160.3
68.4
580852
583737.60
2885.6
207613517.4
85.5
731256
731140.00
-116.0
200366856.0
102.6
881258
878542.40
-2715.6
308163981.2
Residual sum of squares
75474769.6
Trend line equation y = 8620x - 5872