Concentration in µg/ml

Mean area Response achieved

Response calculated thru Trend line equation

Residual (Response practical -Response theoretical)

Residual square

203.2

2245986

2236041.20

-9944.8

98899047.0

304.8

3361852

3360787.50

-1064.5

1133160.3

406.4

4471125

4485533.80

14408.8

207613517.4

508

5596125

5610280.10

14155.1

200366856.0

609.6

6752581

6735026.40

-17554.6

308163981.2

Residual sum of squares

816176561.9