Parameter |
Quantitative Assay |
Mobile phase |
5.0 g sodium 1-heptanesulfonate monohydrate in 250 ml HPLC grade water, 50 ml glacial acetic acid and 650 ml HPLC grade MeOH. |
Gradient |
100% mobile phase A |
Column |
C18, 5µ, 4.6 x 150 mm |
Guard Column |
C18, 4 × 3 mm |
Flow Rate |
2.0 ml/min |
Run time |
20 min |
Injection Volume |
10 µL |
Column temperature |
20°C |
Detection Wavelength |
330 nm |
Autosampler Temperature |
6°C |
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