Methanol (%)
L-leucineRT (min)
C
1
RT (min)
C
1a
RT
(min)
C
2a
RT (min)
C
2
RT (min)
% Target (GS)
% Target
(L-leucine)
65.9
2
4
13
18
22
100
88
68.4
2
3
9
12
14
98
88
70.9
2
2
6
8
9
97
87
Table 4:
Robustness of the assay following changes in methanol composition in the mobile phase.