Glyburide and its metabolites HLM 30 min MLM 30 min RLM 30 min DLM 30 min MoLM 30 min Glyburide 26.92 57.02 67.95 75.94 56.64 M1 27.62 2.32 3.21 8.96 5.26 M2a 6.67 4.23 1.72 1.17 2.33 M2b 7.59 3.74 1.69 1.12 2.21 M3 5.02 5.10 1.99 1.50 3.02 M4 3.84 1.12 6.54 7.89 5.08 M5 12.14 14.01 11.82 3.36 19.05 M6 5.29 10.04 4.77 1.72 5.95 M7 1.23 1.51 BDL BDL BDL M8 0.91 BDL BDL BDL BDL M9 2.50 BDL BDL BDL BDL BDL-Below the detection limit HLM-Human Liver Microsomes MLM-Mouse Liver Microsomes RLM-Rat Liver Microsomes DLM-Dog Liver Microsomes MoLM-Monkey Liver Microsomes

Table 1: Percentage of metabolites identified after 30 min incubation of glyburide with liver micrsomes from human, mouse, rat, dog and monkey. Drug to metabolite ratio after 30 min is calculated by considering glyburide in zero minute samples as one hundred percent. Incubation and analysis was done in triplicate and the results are within the statistical error limit (P<0.05).