Parameters TLC method RP-HPLC method
ALP BENZ ALP BENZ
L.P Mixturesa 98.71 ± 0.940 99.19 ± 0.938 98.68 ± 1.196 100.39 ± 1.285
Alloben® tabletsb
(B.No.100251)
99.40 ± 1.447 98.66 ± 0.842 100.47 ± 0.954 98.46 ± 0.565
Standard additiona 99.72 ± 0.569 98.97 ± 0.519 97.96 ± 1.107 98.93 ± 0.543
Degree of freedomc
F-test
2.454
(2.787)*
1.954
(2.450)*
0.496
(2.210)*
1.241
(2.357)*
Degree of freedomc
Student’st- test
4.214
(4.818)*
3.247
(4.224)*
2.097
(4.245)*
3.846
(3.971)*
aAverage of 3 determinations
bAverage of 6 determinations
cFigures between parenthesis represent the corresponding tabulated values of t and F at P = 0.05.
cHPLC method using: phosphate buffer (pH=4.0)-acetonitrile- methanol (50: 30: 20, v/v/v) as a mobile phase and 250 nm as the detection wavelength.
Table 1: Determination of the studied drugs in the laboratory prepared mixtures (L.P.) and pharmaceutical preparation by the proposed methods and statistical comparison with the reported RP-HPLC methods.